AffiPCR® Enterovirus 71 Type Real Time PCR

Target Disease Type: Neurological Infections

Specific Application: Enterovirus/Poliovirus

Storage and Shipping : 4 weeks

Enterovirus 71-Type RT-PCR V64-50FRT DataSheet

INTRODUCTON

Enterovirus 71 (EV71) is a virus of the genus Enterovirus in the Picornaviridae family notable for its etiological role in epidemics of severe neurological diseases in children. This virus is a member of the enterovirus species A and appears to have evolved only recently with the first known strain isolated in 1965. It has since spread to various countries in Europa (Bulgaria, Hungary) and Asia (Malaysia, Korea, China, Taiwan, Cambodia) where it has been responsible for several outbreaks. To make a definite diagnosis of EV71 infection, laboratory testing such as PCR or culture for the virus from samples of throat secretions, faeces or CSF (cerebrospinal fluid) is needed.

INTENDED USE

ENTEROVIRUS-71 Real-TM is a Real-Time test for the qualitative detection of Enterovirus-71 Virus RNA in the biological materials and in the environment. ENTEROVIRUS-71 RNA is extracted from specimens, amplified using RT-amplification and detected using fluorescent reporter dye probes specific for Enterovirus-71 or Internal Control (IC) .

PRINCIPLE OF ASSAY

Enterovirus-71 virus detection by the polymerase chain reaction (PCR) is based on the amplification of the pathogen genome specific region by using specific primers. In real-time PCR, the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes that bind specifically to the amplified product. The real-time monitoring of the fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening of the reaction tubes after the PCR run. ENTEROVIRUS-71 Real-TM Qual PCR kit is a qualitative test that contains the Internal Control (IC), which must be used in the extraction procedure in order to control the extraction process of each individual sample and to identify possible reaction inhibition. ENTEROVIRUS-71 Real-TM Qual PCR kit uses “hot[1]start”, which greatly reduces the frequency of nonspecifically primed reactions. “Hot-start” is guaranteed by separation of nucleotides and Taq-polymerase by using a chemically modified polymerase (TaqF), which is activated by heating at 95 °C for 15 min.