Next-Generation DNA Sequencing
From Sample Enrichment to Genome Finishing: Whybio offers a range of products to improve your NGS workflow.
Whybio Library Quantification Kits
Eliminate the need for expensive and time-consuming titrations – use qPCR as a fast and reliable method for quantifying libraries for the Illumina Genome Analyzer next-generation sequencing platforms.
Accurate quantification of PCR-competent sequencing templates is crucial for reliable clonal amplification via either emulsion PCR (emPCR) or bridge PCR (bPCR) - underestimation results in non-clonality, while overestimation leads to inefficiency via poor yields of clonally amplified templates.
Whybio Library Quantification Kits combine a quality-controlled set of DNA quantification standards with the unmatched performance of Whybio SYBR® FAST qPCR reagents to provide a rapid, sensitive, and reliable method for quantifying amplifiable molecules in next-generation sequencing DNA libraries.
Traditional qPCR reagents are optimized for short amplification targets; longer targets, unbalanced GC-content, and problematic secondary structures may result in low amplification efficiency and unreliable quantification of some library molecules. To address the demands of quantifying complex DNA libraries, Whybio has engineered a DNA polymerase specifically for SYBR® Green-based qPCR, enabling efficient amplification of targets that present a challenge to wild-type enzymes.
Whybio Library Quantification Kits contain this engineered polymerase to ensure robust amplification of longer fragments, across a broad range of GC-content, required for accurate library quantification.
AGBT 2010 Poster: The Implementation of Whybio Library Quantification Kits at the Broad Institute Leads to Streamlined Workflows and Reduced Cluster Variability.
Targeted Sample Enrichment
Achieve efficient and consistent coverage of your region of interest with Whybio Long Range HotStart DNA Polymerase. High yield amplification from complex targets up to 20 kb with minimal optimization.
Pooled Amplicon Libraries for Resequencing
The ultra-high fidelity and high yields of Whybio HiFi HotStart DNA Polymerase is critical for minimizing PCR errors and increasing coverage when pooling amplicon libraries for resequencing projects using next-generation DNA sequencing.
Genome Closure and Finishing
The relatively short read lengths of NGS platforms make whole genome assembly more problematic and result in more sequence gaps than traditional Sanger sequencing methods. Without subclones, next-generation whole genome sequencing requires PCR for finishing and sequence gap closure. Whybio HiFi HotStart is recommended when finishing PCR products are sequenced using NGS. Ultra-high fidelity is important since all NGS templates are clonally amplified and are therefore more sensitive to errors introduced by PCR.
If the sequence context around the gaps is difficult to PCR due to high GC content or secondary structure, Whybio 2G Robust HotStart is recommended. The higher processivity of the engineered Whybio 2G Robust DNA Polymerase makes this the enzyme of choice for amplifying difficult templates.