RNA analysis using next-generation sequencing (RNA-Seq) enables significantly higher resolution transcriptome profiling compared to other methods such as Sanger sequencing and microarrays. RNA-Seq applications include:
- Gene expression profiling
- Identification of novel transcripts, isoforms and gene fusions
- Alternative splice sites
- Rare transcripts
- Coding SNPs (cSNP)
Depending on the enrichment or depletion strategy, RNA-Seq can be used to evaluate different populations of RNA, including total RNA, non-coding RNA (e.g microRNA, lncRNA, etc.) and mRNA.
Solix Stranded RNA-Seq Kits include all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing. The kits provide precise measurement of strand orientation, uniform coverage, and high-confidence mapping of alternate transcripts.
Solix Stranded mRNA-Seq Kits contain all the reagents in the Solix RNA-Seq kit and the Solix mRNA Capture Beads for the isolation of poly(A)-tailed RNA to analyze novel transcripts and isoforms, alternative splice sites, rare transcripts, and SNPs.