AffiPCR® Rotavirus/Norovirus/Astrovirus Real Time PCR

Target Disease Type: Intestinal Infections

Specific Application: Rotavirus/Norovirus/Astrovirus

Storage and Shipping : 4 weeks

Rotavirus/Norovirus/Astrovirus RT-PCR (CE) V40-50FRT DataSheet

INTRODUCTION

Acute Intestinal Infection (A.I.I.) are one of the primary causes of hospitalization in infectious disease departments. In accordance with the data provided by the contemporary literature, the most often detectable and generally spread etiological agents of A.I.I. are bacterial microorganisms such as Shigella spp. and enteroinvasive E. coli (EIEC), Salmonella spp., thermophilic group of Campylobacter spp., enteropathogenic E.coli (EPEC) and enteroaggregative E. coli (EAEC) and viral agents such as group A rotaviruses, genotype 2 noroviruses, group F adenoviruses (type 40 and 41) and astroviruses.

INTENDED USE

Kit Rotavirus/Norovirus/Astrovirus Real-TM is a Real-Time test for the qualitative detection and differentiation of Rotavirus A, Norovirus 2 genotype, Astrovirus in the biological materials and in the environment. RNA is extracted from specimens, amplified using RT-amplification and detected using fluorescent reporter dye probes specific for Rotavirus/Norovirus/Astrovirus RNA and IC (Internal Control) .

PRINCIPLE OF ASSAY

Rotavirus/Norovirus/Astrovirus Real-TM Test is based on three major processes: isolation of virus RNA from specimens, one-step reverse transcription of the RNA and Real Time amplification of the cDNA. Rotavirus/Norovirus/Astrovirus detection by the polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific primers and detection via fluorescent dyes. These dyes are linked with probes of oligonucleotides which bind specifically to the amplified product. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. Rotavirus/Norovirus/Astrovirus Real-TM PCR kit is a qualitative test which contain the Internal Control (IC) . It must be used in the isolation procedure in order to control the process of each individual sample extraction and serves also to identify possible reaction inhibition.