AffiPCR® West Nile Virus Real Time PCR

Target Disease Type: Dangerous Infections

Specific Application: West Nile Virus

Storage and Shipping : 4 weeks

West Nile Virus RT-PCR (CE) V53-50FRT DataSheet

INTRODUCTION

The West Nile virus is one of the many members of the genus Flavivirus that are known to cause human disease. The life cycle of the West Nile virus involves the microbe’s transmission from nonhuman animals to humans by way of Aedes, Culex, or Anopheles mosquitoes. The West Nile virus can infect horses, birds dogs, and other mammals. However, wild birds are apparently the optimal hosts for harboring and replicating the virus. The West Nile virus causes serious manifestations in approximately 1% of person who are infected, with increased morbidity and mortality in individuals older than 50 years. In hospitalized patients, neurologic sequelae of the West Nile virus included severe muscle weakness, with approximately 10% of patients developing a complete flaccid paralysis. One in 150 West Nile viruses infections results in encephalitis or meningitis, and the mortality rate from severe illness is 3-15%.

INTENDED USE

WNV Real-TM is Real-Time amplification test for the qualitative detection of West Nile Virus (WNV) RNA in clinical specimens (plasma, serum; white blood cells; cerebrospinal fluid), autopsy material of human and animals (brain tissue), biological material (mosquitoes) and water.

PRINCIPLE OF ASSAY

WNV Real-TM Test is based on three major processes: isolation of WNV RNA from specimens, one-step reverse transcription of the RNA and Real Time amplification of the cDNA. WNV detection by the polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific primers and detection via fluorescent dyes. These dyes are linked with probes of oligonucleotides which bind specifically to the amplified product. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. WNV Real-TM PCR kit is a qualitative test which contains the Internal Control (IC) . It must be used in the isolation procedure in order to control the process of each individual sample extraction and serves also to identify possible reaction inhibition.