AffiPCR® Enterovirus Real Time PCR

Target Disease Type: Neurological Infections

Specific Application: Enterovirus/Poliovirus

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Enterovirus RT-PCR (CE) V16-50FRT DataSheet

INTRODUCTION

Enteroviruses are a genus of (+) ssRNA viruses associated with several human and mammalian diseases. Serologic studies have distinguished 66 human enterovirus serotypes on the basis of antibody neutralization tests. Additional antigenic variants have been defined within several of the serotypes on the basis of reduced or nonreciprocal cross-neutralization between variant strains. On the basis of their pathogenesis in humans and animals, the enteroviruses were originally classified into four groups, polioviruses, Coxsackie A viruses (CA), Coxsackie B viruses (CB), and echoviruses, but it was quickly realized that there were significant overlaps in the biological properties of viruses in the different groups. Enteroviruses affect millions of people worldwide each year, and are often found in the respiratory secretions (e.g., saliva, sputum, or nasal mucus) and stool of an infected person.

Historically, poliomyelitis was the most significant disease caused by an enterovirus, Poliovirus. There are 62 non-polio enteroviruses that can cause disease in humans: 23 Coxsackie A viruses, 6 Coxsackie B viruses, 28 echoviruses, and 5 other enteroviruses. Poliovirus, as well as coxsackie and echovirus are spread through the fecal-oral route. Infection can result in a wide variety of symptoms ranging from mild respiratory illness (common cold), hand, foot and mouth disease, acute hemorrhagic conjunctivitis, aseptic meningitis, myocarditis, severe neonatal sepsis-like disease, and acute flaccid paralysis.

INTENDED USE

Kit Enterovirus Real-TM is a Real-Time test for the qualitative detection of Enterovirus RNA in the biological materials and in the environment. RNA is extracted from specimens, amplified using one step RT-amplification and detected using fluorescent reporter dye probes specific for Enterovirus RNA and IC (Internal Control) .

PRINCIPLE OF ASSAY

Kit Enterovirus Real-TM is based on three major processes: isolation of RNA from specimens, reverse transcription of the RNA and Real Time amplification