AffiPCR® Obesity & Diabetes Genetic Screening Real Time PCR

Real Time PCR test for dection of 8 single nucleotide polymorphisms (SNPs) : PPARG2, ADRB2, ADRB3, FABP2, LPL, INS and FTO genes

Storage & Shipping :

Stable for up to one year when stored at –20°C, store it at –70°C or below for longer periods.

Obesity & Diabetes Screen T01372-96-S DataSheet

INTRODUCTION

Obesity is one of the most prevalent disorders of developed countries, and its incidence is increasing rapidly. Obesity in general is associated with physiological changes that may cause the development of diseases like high blood pressure, heart disease, high blood cholesterol and type 2 diabetes.

Diabetes is defined as the inability of the body to regulate and control the blood sugar levels. Persistent and long term raised blood sugar is associated with damage to blood vessels, nerves and several organs.

INTENDED USE

Obesity & Diabetes Screen kit is intended for detection and allelic discrimination of genetic polymorphisms associated with susceptibility to increased risk of diseases associated with obesity and diabetes.

PRINCIPLE OF ASSAY

Obesity & Diabetes Screen kit is a qualitative test that allows the detection by Real Time PCR based on the amplification of the genome specific region using specific primers. In Real Time PCR the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes that bind specifically to the amplified product. The real-time monitoring of the fluorescence intensities during the reaction allows the detection of accumulating product without re-opening of the reaction tubes after the PCR run. One dye (HEX) will detect wild type sequence, the other one (FAM) the mutant sequence. If a patient has positive signal in both fluorescence channels, it means it’s heterozygous genotype. If patient has positive signal only in FAM channel, will be considered carrier of mutant genotype for the target gene/region. If patient has positive signal only in HEX channel, will be considered normal (wild-type) genotype for the target gene/region.