AffiPCR® One Step qRT-PCR SYBR Green Kit V2

AffiPCR® One Step qRT-PCR SYBR Green Kit V2

AffiPCR® One Step qRT-PCR SYBR Green Kit V2 is specially designed for qPCR detection using RNA (e.g., RNA virus) as templates. Reverse transcription and qPCR can be finished in one tube in the presence of Gene Specific Primers (GSP). There is no additional opening/pipetting operations are required, which greatly increasing assay throughput and reducing the risk of contamination. Intergrating the superior performance of HiScript II Reverse Transcriptase and Champagne Taq plus DNA Polymerase with the optimized buffer, the lowest RNA template for detection of this kit is 0.1 pg. The specificity-enhancing factor existing in the buffer can effectively reduce the formation of primer dimers and improve the specificity of product. The kit is provided as the formation of master mix, which is convenient to use.

Storage:

Store at -30°C ~ -15°C and transport at ≤0°C. Store 2X One Step SYBR Green Mix at -30°C ~ -15°C and protect from light.

Applications:

It is applicable for detection of various RNA nucleic acids of animals, plants and microorganisms (viruses, etc.).

Notes:

For research use only. Not for use in diagnostic procedures.

1. The One Step SYBR Green Enzyme Mix contains high concentration of glycerol. Please centrifuge briefly and mix gently before use.

2. When using 2X One Step SYBR Green Mix, please mix thoroughly and pipett accurately, avoid strong light exposure, and protect from light.

3. To avoid contamination, please use RNase-free tips and EP tubes.

Equivalence: