PCR

PCR

Polymerase

TAQ Name Reference
Vial Reactions

Rapid PCR solutions 

AffiPCR 2 X Rapid Taq Master Mix
AFG-VZ-P222-01
5 mL (200 Recations)
AFG-VZ-P222-02
15 mL (600 Recations)
AFG-VZ-P222-03
50 × 1 mL (2000 Recations)
AFG-VZ-P222-04
10 × 5 mL (2000 Recations)

High-Fidelity PCR polymerases

AffiPCR® Super-Fidelity DNA Polymerase
AFG-VZ-P501-d1
100 U
AFG-VZ-P501-d2
500 U
AFG-VZ-P501-d3
1,000 U
AffiPCR® EVO HS Super-Fidelity DNA Polymerase AFG-VZ-P504-d1
100 U
AffiPCR® Max Super-Fidelity DNA Polymerase
AFG-VZ-P505-d1
100 U
AFG-VZ-P505-d2
500 U
AFG-VZ-P505-d3
1,000 U
AffiPCR® 2X Flash Master Mix
AFG-VZ-P510-01
1 mL
AFG-VZ-P510-02
5 × 1 mL
AFG-VZ-P510-03
15 × 1 mL
AffiPCR 2X Max Master Mix
AFG-VZ-P515-01
1 ml
AFG-VZ-P515-02
5 × 1 mL
AFG-VZ-P515-03
15 × 1 mL
AffiPCR® 2X Flash Master Mix (Dye Plus)
AFG-VZ-P520-01
1 mL
AFG-VZ-P520-02
5 × 1 mL
AFG-VZ-P520-03
15 × 1 mL
AffiPCR® 2X Max Master Mix (Dye Plus)
AFG-VZ-P525-01
1 mL
AFG-VZ-P525-02
5 × 1 ml
AFG-VZ-P525-03
15 × 1 ml
AffiPCR® 2X Master Mix (Dye Plus)
AFG-VZ-PK511-01
1 mL
AFG-VZ-PK511-02
5 x 1 mL
AFG-VZ-PK511-03
15 x 1 mL

PCR (Classic)

AffiPCR® Taq DNA Polymerase (Mg2+ plus Buffer)
AFG-VZ-P101-01
1,000 U
AFG-VZ-P101-03
10,000 U
AffiPCR® 2X Taq Master Mix
AFG-VZ-P111-01
5 × 1 mL
AFG-VZ-P111-02
15 × 1 mL
AFG-VZ-P111-03
50 × 1 mL
AffiPCR® 2X Taq Master Mix (Dye Plus)
AFG-VZ-P112-01
5 × 1 mL
AFG-VZ-P112-02
15 × 1 mL
AFG-VZ-P112-03
50 × 1 mL
AffiPCR® 3G Taq Master Mix for PAGE (Red Dye)
AFG-VZ-P115-01
5 mL
AFG-VZ-P115-02
10 × 5 mL
AffiPCR® Green Taq Mix
AFG-VZ-P131-01
5 x 1 mL
AFG-VZ-P131-02
15 x 1 mL
AFG-VZ-P131-03
50 x 1 mL

High-Yield PCR Polymerases

AffiPCR 2 X Taq Plus Master Mix
AFG-VZ-P211-01
5 × 1 mL
AFG-VZ-P211-02
15 × 1 mL
AFG-VZ-P211-03
50 × 1 mL
AffiPCR® 2X Taq Plus Master Mix II (Dye Plus)
AFG-VZ-P213-01
5 × 1 mL
AFG-VZ-P213-02
15 × 1 mL
AFG-VZ-P213-03
50 × 1 mL

Long-Range PCR Kits

AffiPCR® 2X LAmp Master Mix
AFG-VZ-P311-01
1 mL
AFG-VZ-P311-02
5 × 1 mL
AFG-VZ-P311-03
15 × 1 mL
AffiPCR® 2X LAmp Master Mix (Dye Plus)
AFG-VZ-P312-01
1 mL
AFG-VZ-P312-02
5 × 1 mL
AFG-VZ-P312-03
15 × 1 mL

Direct PCR Kits

AffiPCR® One Step Mouse Genotyping Kit AFG-VZ-PD101-01
200 Reactions (50 µL/Reaction)
AffiPCR® Blood Direct PCR Kit V2
AFG-VZ-PD103-01
50 Reactions (50 µL/Reaction)
AFG-VZ-PD103-02
200 Reactions (50 µl/Reaction
AffiPCR® Plant Direct PCR Kit AFG-VZ-PD105-02
200 Reactions (50 µL/Reaction)

PCR-Related Reagents

AffiPCR® PCR Enhancer AFG-VZ-P021-01
500 μL
AffiPCR® dNTP Mix (10 mM each)
AFG-VZ-P031-01
1 mL
AFG-VZ-P031-02
5 × 1 mL
AffiPCR® dUTP (100 mM) AFG-VZ-P033-01
1 mL
AffiPCR® dATP (100 mM) AFG-VZ-P034-01
1 mL
AffiPCR® dTTP (100 mM) AFG-VZ-P035-01
1 mL
AffiPCR® dCTP (100 mM) AFG-VZ-P036-01
1 mL
AffiPCR® dGTP (100 mM) AFG-VZ-P037-01
1 mL

Fast Cloning kits

AffiCLONE® II One Step Cloning Kit
AFG-VZ-C112-01
25 Reactions (20 µL/Reaction)
AFG-VZ-C112-02
50 Reactions (20 µL/Reaction)
AffiCLONE® MultiS One Step Cloning Kit
AFG-VZ-C113-01
10 Reactions (20 µL/Reaction)
AFG-VZ-C113-02
25 Reactions (20 µL/Reaction)
AffiCLONE® Ultra One Step Cloning Kit
AFG-VZ-C115-01
25 Reactions
AFG-VZ-C115-02
50 Reactions
AffiCLONE® ClonExpress Ultra One Step Cloning Kit V2
AFG-VZ-C116-01
20 Reactions
AFG-VZ-C116-02
40 Reactions

Rapid Site-Directed Mutagenesis kits

AffiGEN® Express II Fast Mutagenesis Kit V2
AFG-VZ-C214-01
10 Reactions (20 µL/Reaction)
AFG-VZ-C214-02
25 Reactions (20 μl/Reaction)
AffiGEN® Express MultiS Fast Mutagenesis Kit V2
AFG-VZ-C215-01
10 Reactions (20 µL/Reaction)
AFG-VZ-C215-02
25 Reactions (20 μl/Reaction)

Traditional/TA Cloning Mixes

AffiZYME® T4 DNA Ligase AFG-VZ-C301-01
40,000 U
AffiCLONE® 5 min Universal Ligation Mix
AFG-VZ-C311-01
50 Reactions (10 µL/Reaction)
AFG-VZ-C311-02
100 Reactions (10 µL/Reaction)

TOPO Cloning

AffiCLONE 5 min TA/Blunt-Zero Cloning Kit
AFG-VZ-C601-01
25 Reactions (5 µL/Reaction)
AFG-VZ-C601-02
50 Reactions (5 µL/Reaction)
AffiCLONE® 5 min TOPO-Blunt Cloning Kit
AFG-VZ-C602-01
20 Reactions (2 µL/Reaction)
AFG-VZ-C602-02
40 Reactions (2 µL/Reaction)
AffiCLONE® Ultra-Universal TOPO Cloning Kit
AFG-VZ-C603-01
20 Reactions (10 μll/Reaction)
AFG-VZ-C603-02
40 Reactions (10 μll/Reaction)

GelRed

AffiDYE® GelRed (10,000X)
AFG-VZ-GR501-01
0.5 mL
AFG-VZ-GR501-02
10 × 0.5 mL
AFG-VZ-GR501-03
100 × 0.5 mL

Marker

AffiDYE® DL2000 Plus DNA Marker
AFG-VZ-MD101-01
50 Reactions (5 µL/Reaction)
AFG-VZ-MD101-02
100 Reactions (5 µL/Reaction)
AffiDYE® DL5000 DNA Marker
AFG-VZ-MD102-01
50 Reactions (5 µL/Reaction)
AFG-VZ-MD102-02
100 Reactions (5 µL/Reaction)
AffiDYE® DL15000 DNA Marker
AFG-VZ-MD103-01
50 Reactions (5 µL/Reaction)
AFG-VZ-MD103-02
100 Reactions (5 µL/Reaction)
AffiDYE® 100 bp DNA Ladder
AFG-VZ-MD104-01
50 Reactions (5 µL/Reaction)
AFG-VZ-MD104-02
100 Reactions (5 µL/Reaction)

Universal RT-PCR/RT-qPCR

AffiPCR® 1st Strand cDNA Synthesis Kit V2
AFG-VZ-R211-01
50 Reactions (20 µL/Reaction)
AFG-VZ-R211-02
100 Reactions (20 µL/Reaction)
AffiPCR® 1st Strand cDNA Synthesis Kit (+gDNA wiper) V2
AFG-VZ-R212-01
50 Reactions (20 µL/Reaction)
AFG-VZ-R212-02
100 Reactions (20 µL/Reaction)
AffiPCR® 1st Strand cDNA Synthesis Kit (+gDNA wiper) V3
AFG-VZ-R312-01
50 Reactions (20 µL/Reaction)
AFG-VZ-R312-02
100 Reactions (20 µL/Reaction)

RT-qPCR SuperMix

AffiPCR® RT SuperMix for qPCR V2 AFG-VZ-R222-01
100 Reactions (20 µL/Reaction)
AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V2 AFG-VZ-R223-01
100 Reactions (20 µL/Reaction)
AffiPCR® Select RT SuperMix for qPCR V2 AFG-VZ-R232-01
100 Reactions (20 µL/Reaction)
AffiPCR® Select RT SuperMix for qPCR (+gDNA wiper) V2 AFG-VZ-R233-01
100 Reactions (20 µL/Reaction)
AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V3 AFG-VZ-R323-01
100 Reactions (20 μl/Reaction)
AffiPCR® All-in-one RT SuperMix Perfect for qPCR V3 AFG-VZ-R333-01
100 Reactions (20 μl/Reaction)
AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V4 AFG-VZ-R423-01
100 Reactions (20 µL/Reaction)

Single Cell Sequence

AffiPCR® Single Cell Sequence Specific Amplification Kit AFG-VZ-P621-01
200 Reactions (20 µL/Reaction)

miRNA Reverse Transcription

AffiPCR® miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)
AFG-VZ-MR101-01
50 Reactions (20 µL/Reaction)
AFG-VZ-MR101-02
100 Reactions (20 µL/Reaction)
AffiPCR® miRNA 1st Strand cDNA Synthesis Kit (by tailing A)
AFG-VZ-MR201-01
20 Reactions (20 µL/Reaction)
AFG-VZ-MR201-02
50 Reactions (20 µL/Reaction)

3' RACE and 5' RACE

AffiPCR® 5'/3' RACE Kit AFG-VZ-RA101-01
10 Reactions (20 μl/Reaction)
AffiPCR® 10X Universal Primer Mix (UPM) AFG-VZ-RA102-01
100 Reactions (5 μl/Reaction)
AffiPCR® 2X PCR Mix
AFG-VZ-RA103-01
40 Reactions (25 μl/Reaction)
AFG-VZ-RA103-02
200 Reactions (25 μl/Reaction)

miRNA qPCR

AffiPCR® miRNA Universal SYBR qPCR Master Mix
AFG-VZ-MQ101-01
125 Reactions (20 µL/Reaction)
AFG-VZ-MQ101-02
500 Reactions (20 µL/Reaction)

qPCR Master Mix (SYBR Green)

AffiPCR® SYBR qPCR Master Mix
AFG-VZ-Q311-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q311-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® SYBR qPCR Master Mix (Without ROX)
AFG-VZ-Q321-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q321-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® SYBR qPCR Master Mix (Low ROX Premixed)
AFG-VZ-Q331-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q331-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® SYBR qPCR Master Mix (High ROX Premixed)
AFG-VZ-Q341-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q341-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® SYBR Color qPCR Master Mix
AFG-VZ-Q411-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q411-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® SYBR Color qPCR Master Mix (Without ROX)
AFG-VZ-Q421-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q421-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® SYBR Color qPCR Master Mix (Low ROX Premixed)
AFG-VZ-Q431-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q431-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® SYBR Color qPCR Master Mix (High ROX Premixed)
AFG-VZ-Q441-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q441-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® Universal SYBR qPCR Master Mix Passive ROX
AFG-VZ-Q511-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q511-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® Universal SYBR qPCR Master Mix
AFG-VZ-Q711-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q711-03
2,500 Reactions (20 μl/Reaction)
AffiPCR® Taq Pro Universal SYBR qPCR Master Mix
AFG-VZ-Q712-02
500 Reactions (20 μl/Reaction)
AFG-VZ-Q712-03
2500 Reactions (20 μl/Reaction)

One-Step RT-qPCR Mix

AffiPCR® One Step qRT-PCR SYBR Green Kit V2 AFG-VZ-Q221-01
250 Reactions (20 μl/Reaction)
AffiPCR® One Step qRT-PCR Probe Kit V1 AFG-VZ-Q222-01
250 Reactions (20 μl/Reaction)
AffiPCR® One Step qRT-PCR Probe Kit V2 AFG-VZ-Q223-EN01
250 Reactions (20 μl/Reaction)

Host Cell Residual DNA Quantitative

AffiDETECT® ResiDNA Precise Quantitative CHO DNA Kit AFG-VZ-RD102-01
100 Reactions (30 μl/Reaction)

Cell Apoptosis Detection

AffiDETECT® TUNEL BrightGreen Apoptosis Detection Kit
AFG-VZ-A112-01
25 Reactions
AFG-VZ-A112-02
50 Reactions
AFG-VZ-A112-03
100 Reactions
AffiDETECT® TUNEL BrightRed Apoptosis Detection Kit
AFG-VZ-A113-01
25 Reactions
AFG-VZ-A113-02
50 Reactions
AFG-VZ-A113-03
100 Reactions
AffiDETECT® Annexin V-FITC/PI Apoptosis Detection Kit
AFG-VZ-A211-01
50 Reactions
AFG-VZ-A211-02
100 Reactions
AffiDETECT® Annexin V-PE/7-AAD Apoptosis Detection Kit
AFG-VZ-A213-01
50 Reactions
AFG-VZ-A213-02
100 Reactions

Cell Proliferation Elisa

AffiCELL® CCK-8 Cell Counting Kit
AFG-VZ-A311-01
500 Reactions (10 μl/Reaction)
AFG-VZ-A311-02
1,000 Reactions (10 μl/Reaction)

Dual Luciferase Reporter Assay

AffiASSAY® Dual Luciferase Reporter Assay Kit AFG-VZ-DL101-01
100 Reactions (100 μl/Reaction)

Mycoplasma Detection

AffiDETECT® MycoBlue Mycoplasma Detector
AFG-VZ-D101-01
20 Reactions
AFG-VZ-D101-02
50 Reactions
AffiGEN® Myco-Off Mycoplasma Cleaner
AFG-VZ-D103-01
100 μl
AFG-VZ-D103-02
500 μl
AFG-VZ-D103-03
1,000 μl

Western Blot

AffiGEN® SuperFemto ECL Chemiluminescence Kit
AFG-VZ-E423-01
100 mL
AFG-VZ-E423-02
500 mL

BCA Protein

AffiASSAY® BCA Protein Quantification Kit
AFG-VZ-E112-01
250 Reactions
AFG-VZ-E112-02
500 Reactions

Editing

AffiZYME® T7 Endonuclease I
AFG-VZ-EN303-01
250 U
AFG-VZ-EN303-02
1,250 U

In Vitro Transcription

AffiGEN® T7 High Yield RNA Transcription Kit
AFG-VZ-TR101-01
50 Reactions
AFG-VZ-TR101-02
100 Reactions

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