AffiPCR® CMV/EBV/HHV6 Quantitative Real Time PCR

Target Disease Type: Herpes Virus Infections

Specific Application: Cytomegalovirus (CMV)

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CMV/EBV/HHV6 Quant RT-PCR V48-100FRT DataSheet

INTENDED USE

The CMV/EBV/HHV6 Quant Real-TM is a “Real-Time Amplification” test for the quantitative detection and differentiation of Cytomegalovirus (CMV), Epstein Barr Virus (EBV) and Human Herpes 6 Virus (HHV6) in the biological materials. DNA is extracted from samples, amplified using real time amplification with fluorescent reporter dye probes specific for CMV/EBV/HHV6 and Internal Control (IC) . Test contains an IC (β-globine gene) which allows controlling both PCR-analysis stages (DNA extraction and PCR amplification), material sampling, and storage conditions.

PRINCIPLE OF PCR DETECTION

CMV, EBV and HHV6 detection by polymerase chain reaction (PCR) with hybridization[1]fluorescence detection includes DNA extraction from clinical samples and PCR amplification of pathogen genome specific region with real-time hybridization-fluorescence detection. During DNA extraction from clinical material, human genomic DNA (endogenous internal control) is amplified. Endogenous internal control (IC Glob) allows controlling both PCR-analysis stages (DNA extraction and PCR amplification), material sampling, and storage adequacy. Then, the obtained samples are amplified using specific primers and polymerase (TaqF) . In real-time PCR, the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes which bind specifically to the amplified product during thermocycling. The real-time monitoring of the fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening the reaction tubes after the PCR run.