AffiPCR® Coxiella burnetii Real Time PCR

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AFG-SCC-107
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Description

AffiPCR® Coxiella burnetii Real Time PCR | AFG-SCC-107 | AFFIGEN

Description: Real Time Amplification kit

Target Disease Type: Dangerous Infections

Specific Application: Coxiella burnetii

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Coxiella burnetii RT-PCR (CE) B85-50FRT DataSheet

INTRODUCTION

Q fever is a zoonosis caused by Coxiella burnetii, an obligate gram-negative intracellular bacterium. Most commonly reported in southern France and Australia, Q fever occurs worldwide. C burnetii infects various hosts, including humans, ruminants (cattle, sheep, goats), and pets. In rare cases, C burnetii infection in reptiles, birds, and ticks has been reported. C burnetii is excreted in urine, milk, feces, and birth products. These products, especially the latter, contain large numbers of bacteria that become aerosolized after drying. Coxiella burnetiiis a highly infectious agent that is rather resistant to heat and drying. It can become airborne and inhaled by humans. A single C. burnetii organism may cause disease in a susceptible person. This agent could be developed for use in biological warfare and is considered a potential terrorist threat.

Molecular detection by PCR is unaffected by changes in the infection cycle, and enables rapid, sensitive and specific detection of C. burnetii in a sample.

INTENDED USE

The Coxiella burnetii Real-TM is a “Real-Time Amplification” test for the qualitative detection of C.burnetii in the human and animal biological materials (whole blood, urine, feces, tissue, milk, etc) and in the environment (soil, water) . C.burnetii DNA is extracted from samples, amplified using real time amplification with fluorescent reporter dye probes specific for C.burnetii and Internal Control ( IC) .

PRINCIPLE OF ASSAY

In Coxiella burnetii Real-TM kit there are 2 independent reactions running in parallel in each tube: the first detects specific fragment of C.burnetii and the second detects internal control (IC) DNA which allows excluding unreliable results. Positive result of reference reaction 2 (IC) together with negative results of reactions 1 (C.burnetii) confirm the absence of C.burnetii in a sample. A negative result of both reactions is a sign of PCR inhibition, and this provides a way to avoid false-negative results.

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Additional Information

Size:
50 Tests
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