AffiPCR® EBV Quantitative Real Time PCR

Target Disease Type: Herpes Virus Infections

Specific Application: Epstein-Barr Virus (EBV)

Storage and Shipping : stock

EBV RT-PCR Quant (CE) V9-100FRT DataSheet

INTRODUCTION

EBV is a DNA virus member of herpes family and known to cause mononucleosis. The more severe, albeit rare, result of EBV infection is malignant transformation and cancer development in various forms, including Burkitt’s lymphoma and nasopharyngeal carcinoma, one of the most common cancers in China. Burkitt's lymphoma (BL) is a malignant form of tumor associated with EBV that is endemic to central parts of Africa and New Guinea with an annual incidence of 6–7 cases per 100 000 and a peak incidence at 6 or 7 years of age. The epidemiological involvement of EBV in Burkitt's lymphoma is based on the recognition of the EBV viral genome in tumor cells, associated with an elevated antibody titre against EBV viral capsid antigen (VCA) .

The primary site of Epstein-Barr virus (EBV) infection is the oropharyngeal cavity. Children and teenagers are commonly afflicted usually after oral contact, hence the name “kissing disease”. Based on serology, about 95% of the world adult population has been infected with EBV and, following primary infection, remains lifelong carriers of the virus. EBV infects resting human B-lymphocytes and epithelial cells, multiplies in the latter and establishes latent infection in memory B-lymphocytes. Recent studies have shown that EBV also is associated with B-cell malignancies such as Hodgkin’s lymphoma (HL) and lymphoproliferative disease in immunosuppressed patients, as well as with some T-cell lymphomas and other epithelial tumors such as gastric cancers. These tumors are characterized by the presence of multiple extrachromosomal copies of the viral genome in tumor cells and the expression of part of the EBV genome.

INTENDED USE

EBV Real-TM Quant kit is a Real-Time test for the Qualitative and Quantitative detection of Epstein Barr Virus.

PRINCIPLE OF ASSAY

EBV Real-TM Quant kit is a Real-Time test for the Qualitative and Quantitative detection of Epstein Barr Virus in the biological materials. DNA is extracted from samples, amplified and detected using fluorescent reporter dye probes specific for EBV DNA, Internal Control IC and endogenous IC glob (β-globine gene) .

β-globin gene DNA is a part of human genome DNA and it should be present in an adequate amount in DNA sample, obtained from the cells. There must be no less than 20 000 genomes per sample (DNA from 10 000 cells) . Internal Control (IC), added during the sample preparation from plasma, liquor, amniotic liquid, sputum, bronchial lavages and other cell free or low in DNA content materials, serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition, while endogenous IC (β-globine gene), present in all samples obtained from cells (whole blood, leucocytes, biopsy and autopsy material, saliva, swabs) allows not only to control analysis steps, but also to estimate sample handling and storage. EBV LMP-gene DNA amplification is detected on JOE (Yellow) /HEX/Cy3 channel, the IC glob (β[1]globin gene) DNA amplification is detected on FAM (Green) channel and exogenous Internal Control IC is detected on Rox (Orange) /TexasRed channel.