AffiPCR® HLA B27 Real Time PCR

Real Time PCR kit for detection of HLA-B27 polymorphism

Storage & Shipping :

Must be stored at 2-8°C.The kit can be shipped at 2-8°C but should be stored at 2-8°C immediately on receipt. .

HLA-B27 RT-PCR R116-50FRT DataSheet

INTRODUCTION

Human Leukocyte Antigen (HLA) B27 (subtypes B*2701-2759) is a class I surface antigen encoded by the B locus in the major histocompatibility complex (MHC) on chromosome 6 and presents antigenic peptides (derived from self and non-self antigens) to T cells. HLA-B27 is strongly associated with ankylosing spondylitis (AS), and other associated inflammatory diseases referred to collectively as "spondyloarthritis".

The first human leukocyte antigen (HLA) haplotype association with inflammatory disease was discovered in 1972, correlating HLA-B27 with ankylosing spondylitis. This remains one of the strongest known associations of disease with HLA-B27. Since then, more than 100 disease associations have been made, including many ocular diseases and systemic diseases with specific ocular manifestations.

According to the data published in the international ImMunoGeneTics database, 45 subtypes of HLA-B27 have been detected. A strong association has been found between the subtypes B*2705, B*2704,B*2702, and B*2707 and AS, whereas other subtypes are not associated with AS.

Various methods have been developed for the identification of the HLA-B27 allele. The PCR[1]based HLA-typing methods, including the standard PCR with sequence-specific primers (SSP), have become widely used alternatives to serologic methods in clinical practice; however, these techniques are time-consuming and laborious. The drawbacks of the current methods, such as long processing time and the requirement for post-PCR manual procedures, have been overcome by the introduction of real-time PCR.

INTENDED USE

HLA-B27 Real-TM is a Real-Time amplification test for the detection of HLA-B27 in the biological materials. HLA-B27 Real-TM kit is designed to assist in the diagnosis of patients with suspected ankylosing spondylitis (AS) and other autoimmune diseases. The test is not intended for tissue typing.

PRINCIPLE OF ASSAY

HLA-B27 Real-TM Test is based on two major processes: isolation of genomic DNA from specimens and Real Time amplification with allele specific primers. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. HLA-B27 Real-TM PCR kit is a qualitative test which contains the Internal Control IC (beta-globine gene), which allows to control the presence of cellular material in the sample.