AffiPCR® Reverse Transcriptase V2

AffiPCR® Reverse Transcriptase V2

AffiPCR® Reverse Transcriptase V2 is a new reverse  transcriptase obtained by in vitro molecular evolution technology on the basis of M-MLV(RNase H-)Reverse Transcriptase. Compared with the previous generation of HiScript Reverse Transcriptase, HiScript II Transcriptase has greatly improved thermal stability, with a half-life of more than 4 h at 50℃, and can remain stable for a long time at 55℃. It is suitable for reverse transcription of RNA templates with complex secondary structures for complex secondary structures.In addition, HiScript II Transcriptase adds multiple point mutations, which further enhances the template affinity and progression, which greatly improves the synthesis ability of full-length cDNA, and can obtain cDNA up to 20 kb. It has higher tolerance to common reverse transcription inhibitors and is very suitable for reverse transcription reaction of plant tissue RNA rich in polysaccharides and polyphenols.

Storage Conditions:

Store at -30°C ~ -15°C and transport at ≤0°C.

Application:

It is applicable for reverse transcription of animal, plant and microbial RNA.

Source:

A recombinant E. coli strain carrying an improved reverse transcriptase gene cloned from M-MLV.

Unit definition:

One unit (U) is defined as the amount of enzyme that incorporates 1 nmol of dTTP into acid-insoluble material in 10 min at 37℃ with Poly(rA)-Oligo (dT) as the template/primer.

Notes:

For research use only. Not for use in diagnostic procedures. Prevent RNase contamination Please keep the experimental area clean; wear clean gloves and masks during operation; consumables such as centrifuge tubes and pipette tips used in the experiment must be RNase-free.