AffiPCR® Rickettsia conorii Real Time PCR

Target Disease Type: Dangerous Infections

Specific Application: Rickettsia

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Rickettsia conorii RT-PCR H2741-50FRT DataSheet

INTRODUCTION

Rickettsia conorii is a Gram-negative, obligate intracellular bacterium of the genus Rickettsiathat causes human disease called Boutonneuse fever, Mediterranean spotted fever, or other names. It is a member of the spotted fever group, localized in most of the regions bordering on the Mediterranean Sea and Black Sea, Israel, Kenya, and other parts of North, Central, and South Africa, as well as India. The prevailing vector is the brown dog tick, Rhipicephalus

sanguineus.

INTENDED USE

Kit Rickettsia conorii Real-TM is an in vitro nucleic acid amplification test for qualitative detection and identification of Rickettsia conorii in the biological materials (blood, eschar swabs, cerebrospinal fluid, ticks, tissue: autopsy, biopsy material) .

PRINCIPLE OF ASSAY

Kit Rickettsia conorii Real-TM is based on two major processes: isolation of DNA from specimens and Real Time amplification. Rickettsia conorii DNA and Internal Control (IC) DNA are extracted from the specimens, amplified using Real-Time amplification and detected by fluorescent reporter dyes linked to hydrolysis probes specific for Rickettsia conorii DNA and IC. IC serves as an extraction and amplification control for each individually processed specimen and to identify possible reaction inhibition. IC is detected in a channel other than the Rickettsia conorii.