AffiPCR® Streptococcus pyogenes Quantitative Real Time PCR

Target Disease Type: Pyogenic infections

Specific Application: Pseudomonas aeruginosa

Storage and Shipping : 6 weeks

Streptococcus pyogenes RT-PCR Quant (CE) B82-100FRT DataSheet

INTRODUCTION

Streptococcus pyogenes is a spherical, Gram-positive bacterium that is the cause of group A streptococcal infections. S. pyogenes displays streptococcal group A antigen on its cell wall. S. pyogenes typically produces large zones of beta-hemolysis when cultured on blood agar plates, and are therefore also called Group A (beta-hemolytic) Streptococcus (abbreviated GABHS) .

INTENDED USE

Streptococcus pyogenes Real-TM Quant kit is a Real-Time test for the qualitative and quantitative detection of S. pyogenes DNA in the clinical materials (swabs, plasma, CSF) by using real-time hybridization-fluorescence detection.

PRINCIPLE OF ASSAY

Streptococcus pyogenes Real-TM Quant kit is a Real-Time test for the Qualitative and Quantitative detection of S. pyogenes in the biological materials. DNA is extracted from samples, amplified and detected using fluorescent reporter dye probes specific for S. pyogenes DNA and Internal Control IC.

Internal Control (IC), added during the sample preparation from plasma, liquor, amniotic liquid and other cell free or low in DNA content materials, serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition.

S.pyogenes DNA amplification is detected on JOE (Yellow) /HEX/Cy3 channel and exogenous Internal Control IC is detected on FAM (Green) channel.