AffiNGS® DNA selection Beads (Superior Ampure XP alternative) for DNA Purification

AffiGEN® AffiNGS®

AffiNGS® DNA selection Beads (Superior Ampure XP alternative)

for DNA Purification

AffiNGS® DNA Selection Beads are SPRI-based magnetic beads designed for DNA purification and fragment size selection during next-generation sequencing (NGS) library preparation.

The beads can be incorporated into compatible DNA and RNA library preparation workflows for nucleic acid cleanup and DNA fragment selection.

Product Overview

AffiNGS® DNA Selection Beads are based on the Solid Phase Reversible Immobilization (SPRI) principle. SPRI magnetic bead technology is widely used for nucleic acid purification, DNA cleanup and fragment size selection.

During NGS library preparation, the beads can be used to purify DNA and perform protocol-defined fragment size selection. The bead-to-sample ratio and reaction conditions influence DNA fragment recovery and selection.

Scientific principle: SPRI-based purification uses controlled binding conditions to reversibly immobilize nucleic acids on magnetic beads, followed by magnetic separation, washing and DNA recovery.

Key Features & Benefits

01

SPRI-Based Technology

Uses Solid Phase Reversible Immobilization for bead-based nucleic acid purification and DNA fragment selection.

 02

DNA Purification

Supports DNA cleanup workflows before downstream molecular biology and sequencing applications.
03

DNA Size Selection

Supports protocol-defined selection of DNA fragments during NGS library preparation.

 04

NGS Compatibility

Compatible with various DNA and RNA library preparation workflows.

SPRI Principle for DNA Purification

Solid Phase Reversible Immobilization is a magnetic bead-based approach for nucleic acid purification. Under suitable chemical conditions, DNA associates with the bead surface.

Magnetic separation allows the beads containing the DNA to be separated from the liquid phase. Washing removes unwanted components before purified DNA is recovered.

1

DNA Binding

Combine the DNA sample with the bead reagent under appropriate binding conditions.
2

Magnetic Separation

A magnetic stand collects the beads while the liquid phase is removed. Washing helps remove unwanted components.
3

DNA Elution

Purified DNA is released from the beads using a suitable elution solution.

Applications

NGS Library Preparation

Supports DNA cleanup and fragment selection during next-generation sequencing library construction.

DNA Fragment Size Selection

Enables protocol-defined selection of DNA fragments for sequencing library preparation.

DNA Library Cleanup

Supports purification of DNA libraries before downstream sequencing steps.

RNA Library Workflows

Can be incorporated into compatible RNA library preparation workflows according to kit instructions.

Product Specifications

Specification Details
Brand AffiGEN®
Product Family AffiNGS®
Product Name AffiNGS® DNA Selection Beads
Catalog Number AFG-YSN-248
Technology Solid Phase Reversible Immobilization (SPRI)
Application DNA purification and DNA size selection
Primary Workflow Next-Generation Sequencing (NGS) library preparation
Product Format Magnetic beads
Available Sizes 5 × 5 mL; 60 mL; 450 mL
Shipping Condition Shipped in a cold pack

Handling & Shipping

  • Product format: Magnetic beads
  • Shipping condition: Shipped in a cold pack
  • Storage: Follow the manufacturer's recommended storage conditions.
  • Handling: Mix and handle the beads according to the applicable product protocol.
  • Workflow: Follow the validated library preparation protocol for bead ratio, incubation, washing and elution.

Frequently Asked Questions

What are AffiNGS® DNA Selection Beads used for?

They are used for DNA purification and DNA fragment size selection, particularly during NGS library preparation.

What does SPRI mean?

SPRI stands for Solid Phase Reversible Immobilization, a magnetic bead-based approach for nucleic acid purification and fragment selection.

Can these beads be used for DNA size selection?

Yes. The product is designed for DNA purification and size selection. DNA fragment recovery depends on the bead-to-sample ratio and protocol conditions.

How are the beads shipped?

AffiNGS® DNA Selection Beads are shipped in a cold pack.

What sizes are available?

Listed product formats include 5 × 5 mL, 60 mL and 450 mL.

Explore AffiNGS® DNA Selection Beads

Discover AffiGEN's SPRI-based solution for DNA purification and size selection in NGS library preparation workflows.

View Product at AffiGEN
21st Sep 2026

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