Mouse IgG Antibody

General description:

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.

Species Reactivity:

Mouse

Specificity:

Detects mouse IgG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with mouse IgG2B, mouse IgG2A, and mouse IgG3 was observed.

Source:

Recombinant Monoclonal Rabbit IgG Clone # 2213C

Purification:

Protein A or G purified from cell culture supernatant

Immunogen:

Mouse IgG

Formulation:

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate:

unconjugated

Grade:

reagent grade

Assay:

≥95% (SDS-PAGE)

Form:

lyophilized powder

Composition:

Protein, ≥80%

Storage temp.:

2-8°C

Packaging:

1, 5, 10 mg in glass bottle

Physical form:

Lyophilized from 0.01 M phosphate buffer, pH 7.2, with 0.015 M NaCl

Application:

Purified mouse IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors.

Disclaimer:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.