AffiPCR® HGV Qualitative Real Time PCR

Target Disease Type: HDV Associated Kits

Specific Application: Hepatitis G (HGV)

Storage and Shipping : 6 weeks

HGV RT-PCR Qual (CE) V2-50FRT DataSheet

INTENDED USE

HGV Real-TM is a Real-Time test for the Qualitative detection of Hepatitis G Virus in human plasma and simultaneous detection of a HGV-specific Internal Control (IC), by dual color detection.

PRINCIPLE OF ASSAY

HGV Real-TM Test is based on three major processes: isolation of virus RNA from specimens, one-step reverse transcription of the RNA and Real Time amplification of the cDNA. HGV detection by the polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific primers and detection via fluorescent dyes. These dyes are linked with probes of oligonucleotides which bind specifically to the amplified product. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. HGV Real-TM PCR kit is a qualitative test which contain the Internal Control (IC) . It must be used in the isolation procedure in order to control the process of each individual sample extraction and serves also to identify possible reaction inhibition.

STABILITY

HGV Real-TM is stable up to the expiration date indicated on the kit label. The product will maintain performance through the control date printed on the label. Exposure to light, heat or humidity may affect the shelf life of some of the kit components and should be avoided. Repeated thawing and freezing of these reagents should be avoided, as this may reduce the sensitivity.