AffiPCR® M-MLV (H-) Reverse Transcriptase

AffiPCR® M-MLV (H-) Reverse Transcriptase

AffiPCR® M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutation. Compared with wild-type M-MLV, the M-MLV (H-) Reverse Transcriptase exhibits higher thermostability and greater processivity, making it a better choice in long cDNA synthesis and high-temperature RT reaction. M-MLV (H-) Reverse Transcriptase possesses terminal nucleotidyl transferase (TdT) activity and can be used in 5' RACE or other experiments.

Storage Conditions:

Store at -30 ~ -15°C and transport at ≤0°C.

Applications:

It is applicable for reverse transcription of animal, plant and microbial RNA.

Source:

A recombinant E. coli strain carrying M-MLV (H-) reverse transcriptase gene.

Unit Definition:

One unit (U) is defined as the amount of enzyme that incorporates 1 nmol of dTTP into acid-insoluble material in 10 min at 37°C with Poly (rA)·Oligo (dT) as the template/primer.

Notes:

For research use only. Not for use in diagnostic procedures. Prevent RNase contamination Please keep the experimental area clean; wear clean gloves and masks during operation; consumables such as centrifuge tubes and pipette tips used in the experiment must be RNase-free.