AffiPCR® One Step RT-PCR Kit (Dye Plus) V2

AffiPCR® One Step RT-PCR Kit (Dye Plus) V2

AffiPCR® One Step RT-PCR Kit (Dye Plus) V2 is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, RNase inhibitor, and Champagne Taq plus hot-start DNA Polymerase, which enables high-sensitive total RNA detection (as little as 1 pg) and long-fragment amplification (as long as 10 kb). The 2X One Step Mix (Dye Plus) contains an opimized buffer, dNTPs, and Loading Dye.

Content of the kit:

a. Contains dNTPs and Loading Dye.

b. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.

Storage:

All components should be stored at -20°C.