AffiPCR® RT SuperMix for qPCR V2

AffiPCR® RT SuperMix for qPCR V2

AffiPCR® Reverse Transcriptase V2 is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The RT SuperMix for qPCR V2 is specially designed for two-step qRT-PCR. The 5X HiScript qRT SuperMix contains all compo-nents required for reverse transcription. With the addition of template RNA and ddH2O, the reaction can proceed quickly. The volume of template RNA can be up to 80% of the total volume, which is very suitable for reverse transcription reaction at low concentration of template RNA. 5X HiScript II qRT SuperMix will not freeze at -20°C.

This product has been specially optimized for qPCR. Oligo (dT) 23VN has stronger anchoring ability for poly A + mRNA than Oligo (dT)18, resulting in higher reverse transcription efficiency. With the optimized proportion of Random primers/Oligo (dT)23VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results. The RT product is compatible with dye-based and probe-based qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.

 Components:

Storage:

Store at -30 ~ -15°C and transport at ≤0°C.

Equivalence: