AffiPCR® RT SuperMix for qPCR V2

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SKU:
AFG-VZ-R222-01
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£248.52
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Description

AffiPCR® RT SuperMix for qPCR V2

AffiPCR® Reverse Transcriptase V2 is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The RT SuperMix for qPCR V2 is specially designed for two-step qRT-PCR. The 5X HiScript qRT SuperMix contains all compo-nents required for reverse transcription. With the addition of template RNA and ddH2O, the reaction can proceed quickly. The volume of template RNA can be up to 80% of the total volume, which is very suitable for reverse transcription reaction at low concentration of template RNA. 5X HiScript II qRT SuperMix will not freeze at -20°C.

This product has been specially optimized for qPCR. Oligo (dT) 23VN has stronger anchoring ability for poly A + mRNA than Oligo (dT)18, resulting in higher reverse transcription efficiency. With the optimized proportion of Random primers/Oligo (dT)23VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results. The RT product is compatible with dye-based and probe-based qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.

 Components:

Component AFG-VZ-R222-01
100 rxns (20 μl/rxn)
RNase free ddH2O 2 × 1 ml
5X HiScript II qRT SuperMixa 400 μl
5X No RT Control Mix b 40 µl

 

a. It contains Buffer, dNTPs, HiScript II Reverse Transcriptase, RNase inhibitor, and Random primers/Oligo (dT)23 VN primer Mix.
b. It does not contain HiScript II reverse transcriptase. Other components are the same as 5 × HiScript II qRT SuperMix for the preparation of No RT Control reaction.

 

Storage:

Store at -30 ~ -15°C and transport at ≤0°C.

Equivalence:

AFG-VZ-R222 AffiGEN AffiPCR® RT SuperMix for qPCR V2
FSQ-201 TOYOBO ReverTra Ace qPCR RT Master Mix
RR036 TaKaRa PrimeScript RT Master Mix (Perfect Real Time)

 

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Additional Information

Size:
100 Reactions (20 µL/Reaction)
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